Protocols
Bead protocols
Preparation of Protein-A - anti-DIG-coated latex beads | 2022.08.19. |
Preparation of Protein-A-antibody-coated latex beads | 2022.08.19. |
Bead-tailed Actin Filaments | 2022.08.16. |
Preparation of Ni-NTA beads | 2022.08.19. |
Preparation of photoreactive beads | 2022.08.19. |
Electrophoresis
Gradient Gels for titin | 2022.08.19. |
Ammoniacal Silver Staining of Electrophoretic gels | 2022.08.19. |
Fluorescent labeling
Fluorescent labeling of actin with Rhodamine-phalloidin | 2022.08.19. |
Citoszkeleton rodamin-falloidin festése (in Hungarian) | 2022.08.18. |
Other
Cleaning of coverslips for Total Internal Fluorescence Microscopy | 2022.08.16. |
DNA end biotinylation for optical tweezers experiment | 2022.08.19. |
Actomyosin in vitro motility assay | 2022.08.19. |
Biokémiai laboratórium oldatai (in Hungarian) | 2022.08.19. |
Protein preparation
Purification of muscle actin I: Preparation of acetone powder | 2022.08.19. |
Isolation of actin from acetone powder | 2022.08.19. |
Preparation of native titin from rabbit longissimus dorsi | 2022.08.19. |
Preparation of myofibrils from glycerinated psoas muscle | 2022.08.16. |
Myomesin Preparation | 2022.08.16. |
Preparation of myosin | 2022.08.19. |
Preparation of Troponin-Tropomyosin (Native tropomyosin) and Reconstitution of Thin Filament (Regulated Actin) | 2022.08.16. |
Papain Mg-S1 preparation | 2022.08.19. |
HMM preparation | 2022.08.19. |
Preparation of and in vitro motility assay on human cardiac myosin | 2022.08.16. |
Preparation of desmin | 2022.08.16. |
Preparation of glycerinated psoas muscle | 2022.08.19. |
Össz hiszton preparálás borjú timuszból (in Hungarian) | 2022.08.16. |