Házi bírálók: Dr. Kiss Balázs, Dr. Molnár Kristóf

 

 

s. Nanosurgery experiments demonstrated characteristic unfolding, deformation, and rupture behaviors of titin filaments consistent with continuum mechanics and force-loading theories. 

Additionally, the study examined muscle-specific myosin-II molecules and their capacity to form physiologically relevant structures. 

In vitro, thick-filament arrangements deviated notably from the typical sarcomeric organization observed in muscles. Although titin and myosin did not spontaneously co-assemble under standard conditions, M-complexes interacted with thick filaments after relaxation. This interaction suggests that titin plays a regulatory rather than purely structural scaffolding role in filament organization.

Further AFM analysis demonstrated the technique’s application in virology by resolving morphological distinctions among SARS-CoV-2 variants. Alpha variants were found to be the most compact, whereas wild-type virions were the largest and most rigid, with delta variants exhibiting intermediate properties. Differences in shape and membrane rigidity among variants suggest evolutionary adaptations influencing viral infectivity and stability.